A peer-reviewed journal published by K. N. Toosi University of Technology

Document Type : Research Article

Authors

1 Radiation Application Department‎, ‎Shahid Beheshti University‎, ‎P.O‎. ‎Box‎ ‎1983969411‎, ‎Tehran‎, ‎Iran

2 Physics and Energy Engineering Department‎, ‎Amirkabir University‎, ‎Hafiz Avenue‎, ‎Tehran‎, ‎Iran

3 Nuclear Physics Department‎, ‎Physics Faculty‎, ‎Shahrood University of Technology, Shahrood, Iran

Abstract

‎The aim of this research was determination of the required time for coagulation of in vivo cut bleeding treated by non-thermal atmospheric pressure plasma‎. ‎To meet this‎, ‎an atmospheric pressure plasma jet device was designed and constructed‎. ‎Helium was used as working gas‎. ‎The electrical parameters and optical emission spectrum of helium plasma were measured‎. ‎The averaged treatment time to coagulate the incision bleeding on the mouse liver was obtained 8.6 μs‎, ‎and the average time of naturally incision bleeding coagulation was 10 min‎.

Highlights

  • The coagulation time of in vivo bleeding treated by cold atmospheric pressure plasma was determined.
  • The optical emission spectrum of plasma showed the existance of ROS and RNS species.
  • The coagulation time of treated cut was compared with control cut.

Keywords

Aleynik, A. N., Baykov, A. N., Baskakov, M. B., et al. (2012). Application of non-equilibrium atmospheric pressure plasma for coagulation and sterilization. In Strategic Technology (IFOST), 2012 7th International Forum on, pages 1–4. IEEE.
Benstaali, B., Boubert, P., Cheron, B. G., et al. (2002). Density and rotational temperature measurements of the OH and NO radicals produced by a gliding arc in humid air. Plasma Chemistry and Plasma Processing, 22(4):553–571.
Benstaali, B., Moussa, D., Addou, A., et al. (1998). Plasma treatment of aqueous solutes: some chemical properties of a gliding arc in humid air. The European Physical Journal-Applied Physics, 4(2):171–179.
Fridman, A., Chirokov, A., and Gutsol, A. (2005). Nonthermal atmospheric pressure discharges. Journal of Physics D: Applied Physics, 38(2):R1.
Fridman, G., Peddinghaus, M., Balasubramanian, M., et al. (2006). Blood coagulation and living tissue sterilization by floating electrode dielectric barrier discharge in air. Plasma Chemistry and Plasma Processing, 26(4):425–442.
Heinlin, J., Morfill, G., Landthaler, M., et al. (2010). Plasma medicine: possible applications in dermatology. JDDG: Journal der Deutschen Dermatologischen Gesellschaft, 8(12):968-976.
Howatson, A. M. (1965). An Introduction to Gas Discharges, 2nd Edition. Pergamon International Library of Science, Technology, Engineering and Social Studies.
Janani, E., Ale-Ebrahim, M., and Mortazavi, P. (2013). In vitro and in vivo studies of the effects of cold argon plasma on decreasing the coagulation time. Iranian Journal of Medical Physics, 10(1):31–36.
Kolb, J. F., Mohamed, A. A. H., Price, R. O., et al. (2008). Cold atmospheric pressure air plasma jet for medical applications. Applied Physics Letters, 92(24):241501.
Laroussi, M., Alexeff, I., and Kang, W. L. (2000). Biological decontamination by nonthermal plasmas. IEEE Transactions on Plasma Science, 28(1):184–188.
Laroussi, M. and Leipold, F. (2004). Evaluation of the roles of reactive species, heat, and UV radiation in the inactivation of bacterial cells by air plasmas at atmospheric pressure. International Journal of Mass Spectrometry, 233(1-3):81–86.
Miyamoto, K., Ikehara, S., Takei, H., et al. (2016). Red blood cell coagulation induced by low-temperature plasma treatment. Archives of Biochemistry and Biophysics, 605:95–101.
Qiu, H., Jin, G., Jin, R., et al. (2007). Analysis of variance of repeated data measured by water maze with SPSS. Journal of Chinese Integrative Medicine, 5(1):101–105.
Stoffels, E. (2002). Biomedical applications of electric gas discharges. High Temperature Material Processes: An International Quarterly of High-Technology Plasma Processes, 6(2).
Uhm, H. S., Lim, J. P., and Li, S. Z. (2007). Sterilization of bacterial endospores by an atmospheric-pressure argon plasma jet. Applied Physics Letters, 90(26):261501.
Weidinger, A. and Kozlov, A. V. (2015). Biological activities of reactive oxygen and nitrogen species: oxidative stress versus signal transduction. Biomolecules, 5(2):472–484.